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KMID : 1123920080220061572
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 6 p.1572 ~ p.1578
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Anti-allergic Effect of Seungmagalgeun-tang through Suppression of NF- and p38 Mitogen-Activated Protein Kinase Activation in the RBL-2H3 Cells
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Lyu Ji-Hyo
Lyu Sun-Ae
Yoon Hwa-Jung
Ko Woo-Shin
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Abstract
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In previous report, Seungmagalgeun-tang (SGT) could exert its anti-inflammatory actions in the BV-2 microglial cells. However, study on the anti-inflammatory effect of SGT in mast cells has not been identified. Therefore, we examined on the anti-inflammatory effect of SGT on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced rat basophilic leukemia (RBL-2H3) cells. SGT inhibited the release of -hexosaminidase and secretion and expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)- and interleukin (IL)-4 on RBL-2H3 cells, without affecting cell viability. The protein expression level of nuclear factor (NF)- (p65) was decreased in the nucleus by SGT. In addition, SGT suppressed the degradation of inhibitory protein protein, the activation of p38 mitogen-activated protein kinase (MAPK), and the expressions of cyclooxygenase (COX)-2 mRNA and protein level in RBL-2H3 cells. These results suggest that SGT could be involved anti-allergic effect by control of NF- (p65) translocation into the nucleus through inhibition of degradation and suppression of COX-2 expression.
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KEYWORD
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Seungmagalgeun-tang, cytokines, nuclear factor kappa B, Inhibitor kappa B, cyclooxygenase-2, mitogen-activaed protein kinases
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